Tuesday, August 25, 2020
What’s Stifling Creativity at Coolburst
Ordinary the world is changing around us. It is a fundamental piece of development, development and adequacy. Everything from the adjustment in climate, an age, government, or religion influence the manner in which individuals view, think and act in specific circumstances and overall in the public eye. History has demonstrated that inventive personalities can eventually improve a result or even the most exceedingly awful. From broadcasts to mobile phones, McIntosh PCs to IPODs and IPADs, it was the associations of these items that faced a significant challenge in putting into someoneââ¬â¢s inventive thought that influence our general public in general. These organizations have seen a lot of benefits lows and highs through the hardest economy periods, yet they kept on driving forward and thrive through such occasions by considering the domain of plausibility and accomplishing their conceivable outcomes. Be that as it may, what happens when an association stifles the idea of rehashing themselves to adjust to changes in the public eye and even the world? Coolburst is encountering a significant hierarchical battle between what worked for them to get them where they are today and the stuff to keep on endeavoring later on. Coolburst is situated in Miami, Florida. The beverage items that they serve are sold in schools and eateries. The customary perspectives on during business have sent them much accomplishment from their beginnings. They have experience extraordinary development as the years progressed, yet as of late, they net revenue has remained consistently with no boosting deals. Executive of Marketing Sam Jenkins has tested Coolburstââ¬â¢s view and the board on changing their perspective and opening their brain to new thoughts under previous CEO Garth LaRoue. Jenkinsââ¬â¢s new thoughts of efficiency and development were viewed as strange inside the association. At last, Jenkins left the Coolburst to go with an organization that was extremely more enhanced and imagination. Seeing these distinctions of assessments among Coolburst and Jenkins is new CEO Luisa Roberado. Presently, Roberado is confronting one of greatest difficulties yet for Coolburst, what changes can be made to make Coolburst increasingly gainful and greater inventiveness to keep up or even outperformed the interest of a consistently evolving society. Was Jenkinsââ¬â¢s new thought that implausible with the association or would he say he was on to something that can change the way Coolburst works together to rival possible contender? This contextual investigation will investigate why Coolburst made some hard memories tolerating Jenkinsââ¬â¢s thought on making Coolburst better later on, what the association can do to keep with social changes, and how Roberado can actualize and even changed the present belief system of Coolburst and help the organization took advantage of their assets and thrive the organization to the top in advancement, inventiveness, and in overall revenues.
Saturday, August 22, 2020
Methodological Rigor Assignment Example | Topics and Well Written Essays - 250 words
Methodological Rigor - Assignment Example Self-choice creates when there is no portrayal of the examples. The self-determination process offers watchfulness to the subjects to have a place with any gathering without appropriate thought of portrayal (Vogt, 2007). Basically, subjects in the examination can choose the gathering to have a place, or people may appoint themselves. The third danger to legitimacy in quantitative research is the volunteer impacts (Vogt, 2007). The danger creates when people don't give agree for them to be examined. The subjects who take an interest in the investigation give distinctive data from the ones who don't give assent. The correspondence among subjects displays numerous entanglements and can broadly adjust data. Development happens because of additional time being spent in an investigation. History impact is a legitimacy danger since it brings about broadened time being outperformed during an exploration study (Christensen et al., 2011). There are different courses through which the intrinsic legitimacy dangers to quantitative research can be moderated. Vogt (2007) takes note of that it is significant for a specialist to individual allot subjects to particular gatherings to dissuade self-determination. Subjects ought to be screened cautiously to keep away from whittling down impact. History impact is a principal issue that can be managed by guaranteeing that the estimations are taken on each interim. Subjects ought not be permitted to control the procedures of an investigation (Vogt, 2007). Measurable force alludes to the capacity of a test to decide with exactness an impact and the validness of its reality (Black, 1999). Measurable force help in decreasing sort I and II mistakes by effectively deciding presence of a relationship or impact (Black, 1999). In a perfect world, it decreases the off base dismissal of an invalid theory in type I blunder (Peck et al., 2011). Thus, it decreases the dismissal of an invalid speculation since it effectively decides the
Friday, July 31, 2020
Summer Jobs Teens and Young Adults are Working (and Saving!)
Summer Jobs Teens and Young Adults are Working (and Saving!) Summer Jobs: Teens and Young Adults are Working (and Saving!) More Than You Think Summer Jobs: Teens and Young Adults are Working (and Saving!) More Than You ThinkYoung people get a bad rap for being lazy, but a new OppLoans survey shows thats not the case at all. Summer is finally here, and while you might think teens and young adults are all gearing up for some fun in the sun, it seems that most of them are using their time off from school to work.This might be a surprising revelation if youve been following recent reporting around teens and summer jobs. In 2015, Pew Research Center published a study on teen employment that found that less than a third of teens ages 16 to 19 worked during summer break in 2014, down from 58 percent in 1978.To their credit, Pew doesnt think laziness is the reason for this decline. Instead, they cite the fact that fewer low-skill, entry-level jobs exist today than they did in decades past and research that shows todays teens are spending their summers volunteering, taking classes, and working unpaid internships at a rate that far outpaces previous generations.But our recent survey on teens, young adults, and summer jobs tells a different story. Of the 1,000 people ages 14-24 who participated in the survey, a whopping 63 percent said they had a job lined up for the summer. Additionally, our data showed that young people with jobs lined up plan to make an average of $4,037 this summer, of which they plan to save an average of 57 percentâ"or $2,301.We broke down what we learned in an infographic, which you can check out below, and went into full analysis mode after the graphic!Where are the jobs for young people?Unsurprisingly, a quarter of the young people who will be working this summer will have a job in the foodservice industry. This makes sense, as restaurants are a good place to find entry-level work as a host, cashier, or server. Retail jobs came in second at 17 percent, then childcareâ"likely nannying, babysitting and tutoringâ"came in third at 11 percent.See the full breakdown below:Food Services: 25%Retail: 17%Childcare: 11%Education: 7%Social/Recreational Service: 2%Administrative: 2%Sales: 5%Hospitality: 2%Entertainment: 2%Journalism: 1%Manufacturing: 4%Construction: 3%Transportation: 1%Agriculture: 2%Finance: 1%Health Services: 4%Utilities: 1%Professional/Business Services: 4%Other: 5%When it comes to job titles, the most commonly reported was cashier, followed by manager, babysitter, and sales associate.What are teens and young adults saving for?With the cost of living and tuition surging to all-time highs across the country, it should come as no surprise that 45 percent of our survey respondents are saving up money from their summer jobs to help pay for school or living expenses.After that, 18 percent of respondents said they are saving up to travel, 17 percent are saving to have some spending money during the school year, and 16 percent plan to use their savings to help support their families.How are young people getting their jobs?Seasoned job hunters know that findin g employment isnt about what you know, its about who you know. And it seems that todays young people are learning this lesson early: 31 percent of survey respondents with summer jobs said they got their position through a friend or family connection. Job hunting sites came in second at 19 percent and social media was third at 11 percent:Job search site: 19%Craigslist: 5%Friend or family connection: 31%Word of mouth: 10%Social media: 11%School resources: 8%Google: 12%Other: 5%Why are so many teens and young adults working?Well, why do YOU work? If your first answer was money, youre in agreement with 33 percent of our survey respondents, who said their main reason for getting a summer job was to generate income and build savings.Learning came next, with 32 percent of young workers reporting the desire to build new skills, and 19 percent said they were looking to make career connections.Forging new friendships was also a popular reason for spending the summer on the grind, with 15 perc ent of survey respondents saying they hoped to find new friends at work.Which regions are leading the charge on summer jobs?While the majority of young people in every part of the country who took our survey said they would have a job this summer, employment rates varied from region to region.Young people in the Midwest are more likely to have a summer job than young people living in any other region. In fact, 69 percent of survey respondents from the Midwest said they will have summer jobs, at which they expect to make an average of $4,251. Young Midwestern workers plan to save, on average, 55 percent of their income, which comes out to an average of $2,338.In the Northeast, 67% of teens and young adults say they will have summer jobs, and they plan to make an average of $3,159 throughout the course of the summer. While Northeastern student workers might expect to make slightly less than their Midwestern peers, theyre also planning to save more of their summer income: 61 percen t, or an average of $1,927.63 percent of survey respondents living out West will have a job this summer. Theyre expecting to make $3,998, of which they plan to save an average of 59 percent, or $2,359.While people in the South are the least likely to have a summer job (with just 57 percent reporting theyll be working this summer) those who are employed are cleaning up. Southern young people who are employed this summer expect to make an average of $4,319â"the highest expected income of any region. Of that $4,319, they expect to save an average of $2,359, or 59 percent.What are teens and young adults WITHOUT jobs doing this summer?While the vast majority of respondents said they would be working this summer, 37 percent of the teens and young adults we surveyed were job-free heading into the summer. So what will these kids be doing with all their free time? We asked and they answered:29 percent say they will be relaxing16 percent say they will be taking classes16 percent will be stud ying and preparing for the upcoming school year13 percent will spend their time volunteering13 percent plan to travel4 percent have an unpaid internshipand 9 percent say they will be doing other, which were taking to mean skydiving lessons. What else could other possibly be?If you enjoyed this piece and want to learn more about earning extra cash, check out these related posts and articles from OppLoans:6 Expert Tips to Start Your Side HustleThe Pros and Cons (and Scams) of Trade SchoolNeed Cash Fast? Try These 10 Great Side HustlesWhat was your summer job as a teen? We want to hear from you! You can email us or you can find us on Facebook and Twitter.
Friday, May 22, 2020
Classification Essay - Three Types of Annoying Shoppers
The Three Types of Annoying Shoppers Tires squeal across the hot asphalt; exhaust fumes fill the air. Innocent shoppers leap to safety as cars circle the neighborhood grocery store lot in search of the ideal parking spot. Engines revving. Adrenaline rushing. The drivers sole mission is to obtain a white lined rectangle of pavement before entering the race known as shopping. As a safety precaution, competent and considerate shoppers should be aware of the distinct character traits of the three annoying types of shoppers: Hurry Harry, Suburban Sally, and Picky Pete. Knowledge of their parking-lot-to-checkout-stand habits increases the chance of avoiding a head-on collision. The first annoying type of shopper, Hurry Harry,â⬠¦show more contentâ⬠¦Words are finger-painted in the dust on the back window above the bumper stickers declaring, My child is an honor student and Vacation at Disneyland. Sticky, diapered youngsters spill from the van to scramble for a rusty cart which desperately needs a front-end alignment. Suburban Sally, in a loose sweatsuit and pink rollers, unravels a grocery list equal to any childÃâ¢s letter to Santa. She skillfully maneuvers the cart through the maze of shoppers, braking without warning, to comparison shop or to threaten her loud bunch of food guzzlers. With the strength of a two-ton truck, she steers her cart to the finish line. As the cashier rings up her purchases, it is time for her to make one last lap around the store for a forgotten item. Customers sigh as she pulls out coupons and rebates to offset the sticker price. She writes a check and calculates the difference before leaving. A good set of brakes and pl enty of tabloids to read are necessary when shopping behind Suburban Sally. In comparison to her absent-minded habits, Picky Pete, the third type of annoying shopper, appears flawless. Sunlight shines off his latest BMW parked across three spaces farthest from the store; his political statements boldly expressed on the shiny chrome bumper: Dont blame me, I voted for Bush and To err is human,Show MoreRelatedLogical Reasoning189930 Words à |à 760 PagesScientific Test ............................................................................................................ 539 Retaining Hypotheses Despite Negative Test Results .............................................................. 540 Three Conditions for a Well-Designed Test ............................................................................... 543 Deducing Predictions for Testing .............................................................................................Read MoreDeveloping Management Skills404131 Words à |à 1617 PagesCONTENTS 60 Core Self-Evaluation Scale 103 Scoring Key 103 Comparison Data 103 2 MANAGING PERSONAL STRESS 105 SKILL ASSESSMENT 106 Diagnostic Surveys for Managing Stress 106 Stress Management Assessment 106 Time Management Assessment 107 Type A Personality Inventory 108 Social Readjustment Rating Scale 109 Sources of Personal Stress 111 SKILL LEARNING 112 Improving the Management of Stress and Time 112 The Role of Management 113 Major Elements of Stress 113 Reactions to Stress 114 CopingRead MoreProject Managment Case Studies214937 Words à |à 860 PagesFems. After two months of meetings, the committee identified the need for three different stage-gate models: one for information systems, one for new products1 services provided, and one for bringing on board new corporate clients. There were several similarities among the three models. However, personal interests dictated the need for three methodologies, all based upon rigid policies and procedures. After a year of using three models, the company recognized it had a problem deciding how to assignRead MoreStephen P. Robbins Timothy A. Judg e (2011) Organizational Behaviour 15th Edition New Jersey: Prentice Hall393164 Words à |à 1573 PagesCoaches for Employees 123 Case Incident 1 Is It Okay to Cry at Work? 124 Case Incident 2 Can You Read Emotions from Faces? 124 S A L S A L 5 Personality and Values 131 Personality 133 What Is Personality? 133 â⬠¢ The Myers-Briggs Type Indicator 135 â⬠¢ The Big Five Personality Model 136 â⬠¢ Other Personality Traits Relevant to OB 139 x CONTENTS Values 144 The Importance of Values 144 â⬠¢ Terminal versus Instrumental Values 144 â⬠¢ Generational Values 145 Linking an Individualââ¬â¢s
Sunday, May 10, 2020
Green Energy is a Substitute Energy Source - 1720 Words
Introduction ââ¬Å"Greenâ⬠(or alternative) energy is a substitute energy source for fossil fuels. Alternative energy is interchangeable with Renewable energy since the sources that are generated are natural and cannot be exhausted, all of which have minimal environmental impact. Alternative energy sources include solar power, which is acquired from the sun; wind power; water energy, which produces hydroelectricity; and biomass energy, which comes from plants. Due to these sources being free and causing no harm to the environment, they are increasingly being used around the world. The energy sector has been growing, as governments are adapting environmental policies to reduce pollution and welcoming alternative energy sources. Alternative energy has many economic, environmental and social benefits. Economically, alternative energy satisfies the continuous and ever-growing demand and cost of energy. As the world populates and new discoveries are made on a daily basis, energy sources are not only being exhausted, they are also harming the environment, and thus people. Due to the high demand for energy and not so high supply, prices are rising day by day. Alternative energies are the solution to this problem, as their cost ranges from nothing to low. Environmentally, switching from fuels to alternative energy saves our world from impacts such as pollution, global warming and deforestation. Socially, our growing dependence on petroleum from around the world can be risky, as supplyShow MoreRelatedIndustry Analysis : The Oil And Gas Industry976 Words à |à 4 PagesIndustry Analysis: The oil and gas industry is expansive and rather complex industry consisting of oil, fossil fuels, natural gas, oil and green energy sources. Combined the above sources make-up 32% of total energy consumed worldwide. Three sectors define the value chain of the oil industry; Upstream ââ¬â which is the process of exploration and extracting the natural resource, control storage and entail refinement process. Midstream ââ¬â consisting of distribution by pipelines and large quantity shipmentsRead MoreE-on Uk Porters 5 Forces1316 Words à |à 6 Pagesin an industrial context, creating a competitive advantage over rival firms. He divided this concept into five separate entities known as the five forces which can be applied to the energy giant E-on. E-on U.K is Britain s second largest multifaceted energy producer, distributor and retailer providing energy to over 45 million customers, part of the worldwide E.ON AG corporation. Rivalry in the power industry is relatively low in terms of production according to the Power Economics (2007)Read MoreEssay On Green Computing1015 Words à |à 5 PagesGreen IT Green IT, also called as green computing, is the study and practice of creating, engineering, using, and disposing of electronic devices such as servers, computers, and associated subsystems ââ¬â like printers, monitors, storage devices, networking, and storage devices, and communications systems ââ¬âeffectively and efficiently with negligible or no impact on the environment. thus, green IT involves software assets, hardware assets, tools, approaches, and practices which can help to enhance andRead MoreThe Benefits of Becoming Green Essay1583 Words à |à 7 PagesImproving Energy How much energy is used from simply charging a cell phone? Harmful fossil fuels are used to produce most of the energy the world uses daily. Although some people believe that fossil fuel industries are stable, enabling green jobs to produce and manage alternate sources of energy would create a more secure, reliable, and healthy economy. What exactly is a green job? ââ¬Å"The term ââ¬Ëgreen jobsââ¬â¢ encompasses a number of fields, ranging from jobs that increase energy efficiency and reduceRead MoreDaily Food Recommendation1302 Words à |à 5 PagesEnglish muffins as I used to, I should have whole wheat English muffins. Also since I regularly have rice with most of my meals, I can substitute brown rice for white rice. Not only brown rice is a good source of energy, but it is also the best source for vitamins. Since I need to increase consuming more food in milk and grains groups, so in order to restraint my energy intake, I probably need to reduce my meat and beans intakes. In order to understand my daily intakes in each category better, I willRead MoreGlobal Warming And Its Effect On The Environment1189 Words à |à 5 Pagesas one of the effective ways. Carbon price is the tax on the emission of carbon into the atmosphere than the decided level as well as it is a tax on energy sources which emit carbon di oxide into the atmosphere. It is effectively a tax on pollution (Wright, 2011). However, some people argue that there could be some ways to change the climate like energy conversation and planting trees, these ways could be good but a carbon taxââ¬â¢s environment effects such as reducing the amount of carbon in the atmosphereRead MoreScientific Evidence For Warming Of The Climate System Essay1662 Words à |à 7 Pagesglobal warming is to create products which are more environmentally friendly. These goods are often called ââ¬Å"naturalâ⬠, ââ¬Å"greenâ⬠, ââ¬Å"eco-friendlyâ⬠, or they carry other eco-labels to tell consumers that they are better for the environment than alternative products. When a consumer walks down the aisle of a shopping market they are bound to see various products that claim to be ââ¬Å"greenâ⬠or ââ¬Å"eco-friendlyâ⬠. Many large retailers including Target, one of the nationââ¬â¢s largest retailers, have exponentially expandedRead MoreGlobal Climate Change And Plan Of Action1485 Words à |à 6 Pageshave signed the United Nations Framework on Climate Change and in the year 2000 our country joined on board with the Kyoto Protocol (Ministry of Ecology). Azerbaijan notes that the economy should shift towards relying on alternate resources towards a green economy. Azerbaijan observes efforts taken by neighboring countries in the attempt to tackle on the issues surrounding global climate change. Azerbaijan will continue to fight to improve the conditions in which our citizens are at risk for. Past andRead MoreThe Future of Nuclear Power941 Words à |à 4 Pagesof power needed is expected to increase, and in recent years there has been a great deal of attention paid to the way in which that power need may be met in a manner that is not detrimental to the environment. One of the more controversial power sources is that of Nuclear power. Nuclear power is know to have the potential to provide relativity long term, high levels of power which does not have the environmental costs associated with the burring of fossil fuels (Chu and Majumdar, 2012). However,Read MoreA Common Misconception Of Fossil Fuels1317 Words à |à 6 Pages Intro Although there is a common misconception that fossil fuels will cause pollution, mankind should rely on fossil fuels for energy not only because they are cheap and plentiful, but also because alternative energy is very unreliable. Coal $0.10 Natural Gas $0.07 Nuclear $0.095 Wind $0.20 Solar PV $0.125 Solar Thermal $0.24 Fossil Fuels are cheap and plentiful. Coal costs 10 cents per kilowatt hour compared to the 12 cents to 24 cents of solar panels
Wednesday, May 6, 2020
Home Care Housing Hrm Free Essays
This report showing the few HR issues currently faced by Home Care Housing. Over 12 years, Home Care Housing Association has employed more than 600 employees. Among those employees, 40% of them will be male and 60% of them will be female. We will write a custom essay sample on Home Care Housing Hrm or any similar topic only for you Order Now This also proven that there will be no proper ratio in workforces between men women. About 60% of female is placed in certain roles and departments such as auxiliary, nursing and office staffs group but none in the managerial and supervise division. This will be considered as one of the gender discrimination. Some units which transferred from local authority will be faced the issue of variation such in levels of pay, hours of work, shift payments and holidays. Head office staffs have increased rates due to cost of living increasing every year but unit staffs none. Unit staffs working hours per week were longer than head office staffs. Even in term of holiday entitlement, head office staffs also entitled more compared to unit staffs. Shift payments are also different in some units due to long service for nursing and auxiliary staffs. Hence proven again the term and conditions in organisationââ¬â¢s policy was not consistent. Because of the strong philanthropic aspects permeating the organisation so that no redundancies happened in the organisation and manager is given too much of autonomy. Some of the managers have simply decided to increase staff pay across the board which based of yearly budgets or sometimes skipping years. The discrimination issue has been noticed while a complaint has been received from a member of staffs mentioned that one of the manager is biased on the good staffs who willing to follow their instructions by giving them monetary advantages like incentive, increment on pay and non-monetary benefits like preferential shift patterns and extra time off. For those staffs whose are refused to comply will get bad shifts and do not have a rise of pay. Irregularity of reward strategy was happened when the cost of living awards only given to those staffs which unit has been transferred from local authority. The payment structure and reward strategy has been introduced but never been considered by them. Furthermore will be the race and religion discrimination issue which one of a family member of residents of one home has viewed the profile of one staff who took care of their family member was from anti-semetic groups and different races from them, so they refused to let that staff to work for their family member. Besides, another discrimination on age issue occurred when one of the manager has posted few deprecating comments about the senior management of the organisation have been corner cutting some of the care standards. For the employees who joined early days are well motivated and most of them were union members. They had the meeting regularly and persuading the others to join as union member. For the other staffs who are non union member who refuse to comply with unionized manager are treated unfairly and have not been motivated by management. On the other hand, another issue will be lack of communication between the older unit management with their staffs. They may have no regular briefing as other units done and always received a lot of complains and grumbles from staffs in the matter of facilities providing and upgrading. In addition, issue of misuse of social media also occurred in the organisation. The organisation had set up an own ââ¬ËFacebookââ¬â¢ page which provided the company information. Although the ââ¬Å"Facebookââ¬â¢ page was successfully attracted some new clients but some negative news like care home abusing and some heated argument which involved of own staffs participation were posted up in the page. Workplace intimidation was happened as one of the family member of one resident has complaint and blamed the cleaning staff has stolen their resident money. They manager of that unit who want to investigate deeply about the case has been threatened by the family member. The organisation has faced the issue of high employee turnover and difficulty on hiring new staffs. The managers found that the applicants were not suitable and even some of the posts have to be advertised so many time before getting the suitable applicants. One of the manager has done for the survey and spoke to the staffs who have resigned from the organisation, realized that those resigned staffs are leaded to other local organisation not only because of they get higher pay than your organisation but other organisation did provided the training and development programme which lead to their position. The organisation did not have proper essentials to meet the standardization of care quality via care quality inspection. Although the organisation did have own policies but didnââ¬â¢t not meet all this standard requirements. As well as the organisation also lack of individual development plans for staffs as a lot of staff did not have certain working skills and poor supervision of managers. Some of the quality manager did not realised the important of having standardisation to care of quality and commission. And some of the quality manager had told their staffs to follow the standard requirements but they did not have proper check after instructing. How to cite Home Care Housing Hrm, Papers
Wednesday, April 29, 2020
The Effect Of Violations Of Hardy-Weinberg Equilibrium Conditions On T
The Effect of Violations of Hardy-Weinberg Equilibrium Conditions on the Allele Frequency of Drosophila melanogaster Populations Introduction The interaction between genetic variation and natural selection is one of the most important concepts in modern biology. The product of this interaction, evolution, which is a change in a population's allele frequency, is responsible for the great complexity and diversity of life seen on earth today. Allele frequencies of a non-evolving population (one in which the allele frequencies are not changing) can be elegantly modeled using the Hardy-Weinberg theorem. For a population to be at equilibrium, five conditions must be met: 1) large population size, 2) random mating, 3) no mutation, 4) no migration, and 5) no natural selection. Violation of these conditions can produce a change in the allele frequencies of the population under study. Our purpose is to examine the effects of small population size and of natural selection. In this experiment, we will use populations of Drosophila melanogaster to model the Hardy-Weinberg theorem. Drosophila populations are ideal for modeling evolutionary dynamics for two reasons: they are inexpensive, to both buy and maintain, and, more important, have a brief reproductive cycle, allowing several generations to be studied in a short length of time. We will be working with two populations of Drosophila, designated A and B. Population A will be used to examine the effects of a small population (the founder effect). Natural selection will be studied using population B. We hypothesize that the violation of a single Hardy-Weinberg equilibrium condition present in each population will give rise to a specific change in allele frequency in that population. We hypothesize that the allele frequencies of the two A sub-populations will diverge over time, due to genetic drift magnified by sampling error in the A-Small population. We hypothesize that the frequency of the Cy allele will decrease in population B, due to natural selection. In order to test our hypotheses, we will be scoring Drosophila for several generations (F2, F3, and F4). We will then be able to calculate allele frequencies within the different populations. These frequencies will be compared to determine if the populations are in Hardy-Weinberg equilibrium and if their alle le frequencies are changing. Materials and Methods For a complete listing of Materials and Methods (including the procedures for anesthetizing and identifying Drosophila), see the Biology 220W Lab Manuel. Population A is comprised of wild-type Drosophila and those marked with one of two mutant traits: eyeless (ey) or sparkling-polished (sp). Each mutation is indicative of a Drosophila that is homozygous for that allele. Heterozygous flies show the wild-type phenotype. The Drosophila in population A are first randomly divided in two groups: a small group, containing 10 flies, and a large group, containing all other flies from population A. Note that the population A-Small must contain at least 2 flies from each sex. The number of Drosophila of each phenotype is determined for both the A-Small and the A-Large. The second and third generations are also scored in this manner. Population B is comprised of the F2 generation of a cross between wild type and curly winged (Cy) Drosophila. All flies with the curly winged phenotype are assumed to be heterozygous, as the Cy mutation is homozygous lethal. The number of flies of each phenotype is determined; this is done for each of three generations. In both parts of the experiment, phenotype frequencies are used to determine allele frequencies. Allele frequencies are used to determine if the populations are in Hardy-Weinberg equilibrium. They are also compared across generations to determine if they are changing. In both parts of the experiment, the independent variable is the small population size (i.e. the violation of Hardy-Weinberg equilibrium) and the dependent variable is the change in allele frequencies. Results Table 1 presents all data for sub-populations A-Small and A-Large. It shows a total number of Wild-type flies in week 5 as 666. Table 2 presents the results of x2 tests performed to test for the existence of Hardy-Weinberg equilibrium within the Drosophila populations. It shows most populations to be far out of Hardy-Weinberg equilibrium. However, all Small sub-populations are in Hardy-Weinberg equilibrium in week 1, at a 0.005 level of confidence. Table 3 shows the tests for shifts in phenotype (which is indicative
Friday, March 20, 2020
A Critique essays
A Critique essays In the book Civil War in the Ozarks, the authors, Phillip W. Steele and Steve Cottrell, describe lifes perils and strategies during the Civil War in the Ozarks of Arkansas and Missouri. Both authors have a rich family background with the Civil War. Phillip W. Steeles family fought on the Confederate side of the war and Steve Cottrells family fought for the Union forces. With both authors having common interest in preserving Ozarks history, they put together a historical review of the Civil War in the Ozarks. In my opinion a Reb and a Yank (respectively) getting together to write on such an event in history is remarkable. Although, I would imagine that is why the book sticks with so many facts, rather than to approach the aspects of the lives involved. The book would have been more enjoyable to read, if they had mingled a bit more of lifes stories within the battle events described. According to the book, Phillip W. Steele and Steve Cottrell have extensively researched the battles that took place between 1861 and 1865 in the writing of this book. They look at the heroes, outlaws, and peacemakers who influenced the role the Ozarks played in the War Between the States. (Back-cover) Before the Civil War abolitionist and pro-slavery forces had been fighting in the Ozarks. This book gives a detailed account of the Civil War battles that followed this era in the Ozarks. Two of the most famous of these battles was the Battle of Pea Ridge (p 48) and the Battle of Prairie Grove (p 60). The skirmishes were more for supplies than territory. The authors touched on the history of Quantrills Raiders and other lives of outlaws that evolved due to the war and violence. (p 56) When the war came to an end, with Robert E. Lees surrender at the Appomattox Court House in Virginia, on April 9,1865 the Union forces were the victors. I found that although ...
Wednesday, March 4, 2020
34 Writing Tips That Will Make You a Better Writer
34 Writing Tips That Will Make You a Better Writer 34 Writing Tips That Will Make You a Better Writer 34 Writing Tips That Will Make You a Better Writer By Daniel Scocco A couple of weeks ago we asked our readers to share their writing tips. The response was far beyond the initial expectations, and the quality of the tips included was amazing. Thanks for everyone who contributed. Now, without further delay, the 34 writing tips that will make you a better writer! 1. Daniel Pay attention to punctuation, especially to the correct use of commas and periods. These two punctuation marks regulate the flow of your thoughts, and they can make your text confusing even if the words are clear. 2. Thomas Participate in NaNoWriMo, which challenges you to write a 50,000 word novel in a month. I noticed that my writing has definitely improved over the course of the book - and itââ¬â¢s not even finished yet. 3. Bill Harper Try not to edit while youââ¬â¢re creating your first draft. Creating and editing are two separate processes using different sides of the brain, and if you try doing both at once youââ¬â¢ll lose. Make a deal with your internal editor that it will get the chance to rip your piece to shreds; it will just need to wait some time. A really nice trick is to switch off your monitor when youââ¬â¢re typing. You canââ¬â¢t edit what you canââ¬â¢t see. 4. Jacinta In a sentence: write daily for 30 minutes minimum! Its easy to notice the difference in a short time. Suddenly, ideas come to you and you think of other things to write. You experiment with styles and voices and words and the language becomes more familiar 5. Ane Mulligan Learn the rules of good writingâ⬠¦ then learn when and how to break them. 6. Pete Bollini I sometimes write out 8 to 10 pages from the book of my favorite writer in longhand. This helps me to get started and swing into the style I wish to write in. 7. Nilima Bhadbhade Be a good reader first. 8. Douglas Davis While spell-checking programs serve as a good tool, they should not be relied upon to detect all mistakes. Regardless of the length of the article, always read and review what you have written. 9. Kukusha Learn to take criticism and seek it out at every opportunity. Dont get upset even if you think the criticism is harsh, dont be offended even if you think its wrong, and always thank those who take the time to offer it. 10. John England Right click on a word to use the thesaurus. Do it again on the new word and make the best use of your vocabulary. 11. Lillie Ammann After editing the work on screen or in print, I like to read the text aloud. Awkward sentences and errors that slipped through earlier edits show up readily when reading out loud. 12. H Devaraja Rao Avoid wordiness. Professor Strunk put it well: ââ¬Å"a sentence should contain no unnecessary words, a paragraph no unnecessary sentences, for the same reason that a drawing should have no unnecessary lines and a machine no unnecessary parts.â⬠13. David Write as if youââ¬â¢re on deadline and have 500 words to make your point. Then do it again. And again. 14. Yvette Sometimes I type in a large font to have the words and sentences bold before me. Sometimes, in the middle of a document I will start a new topic on a fresh sheet to have that clean feeling. Then, Iââ¬â¢ll cut and insert it into the larger document. I wait until my paper is done before I examine my word usage and vocabulary choices. (And reading this column it has reminded me that no two words are ever exactly alike.) So at the end, I take time to examine my choice of words. I have a lot of fun selecting the exact words to pinpoint my thoughts or points. 15. Amit Goyal To be a good writer is to start writing everyday. As Mark Twain said, the secret of getting ahead is getting started. Try using new words. i.e avoid repeating words. this way we learn the usage of different words. Do edit your previous articles. Start with small paragraphs like writing an article for a Newspaper, and proceed from there. 16. John Dodds Remove as many adjectives as possible. Read Jack Finneyââ¬â¢s tale, Cousin Lenââ¬â¢s Wonderful Adjective Cellar for a fantastical tale about how a hack becomes a successful author with the help of a magical salt cellar that removes adjectives from his work. 17. John Ireland I set my writing aside and edit a day or two later with the aim of making it terse. It has trained me to be more conscious of brevity when writing for immediate distribution. 18. Jai Try to write in simple way. Express your views with most appropriate words. 19. Mark Read great writers for inspiration. If you read them enough, their excellent writing style will rub off onto your dazzling blog. YOU ARE what you read (and write!). 20. Caroline I watch my action tense and wordiness in sentences when I am writing my technical diddley. For example, in a sentence where you say â⬠¦Ã¢â¬ you will have toâ⬠¦Ã¢â¬ I replace it with ââ¬Å"â⬠¦you mustâ⬠¦Ã¢â¬ , or ââ¬Å"Click on the Go button toâ⬠¦Ã¢â¬ can be replaced with ââ¬Å"Click Go toâ⬠¦Ã¢â¬ . Think of words such as ââ¬Å"enablesâ⬠, instead of ââ¬Å"allows you toâ⬠or ââ¬Å"helps you toâ⬠. If one word will work where three are, replace it! I always find these, where I slip into conversational as I am writing quickly, then go back and purge, purge, purge. 21. Akhil Tandulwadikar Donââ¬â¢t shy away from adopting the good habits that other writers use. Do not worry about the length of the article as long as it conveys the point. Of course, the fewer words you use, the better. Start the article with a short sentence, not more than 8 words. 22. Julie Martinenza Instead of adding tags (he said/she said) to every bit of dialogue, learn to identify the speaker by showing him/her in action. Example: ââ¬Å"Pass that sweet-smelling turkey this way.â⬠With knife in one hand and fork in the other, Sam looked eager to pounce. 23. Aaron Stroud Write often and to completion by following a realistic writing schedule. 24. Joanna Young One that works for me every time is to focus on the positive intention behind my writing. What is it that I want to communicate, express, convey? By focusing on that, by getting into the state that Iââ¬â¢m trying to express, I find that I stop worrying about the words just let them tumble out of their own accord. Itââ¬â¢s a great strategy for beating writerââ¬â¢s block, or overcoming anxiety about a particular piece of writing, whether thatââ¬â¢s composing a formal business letter, writing a piece from the heart, or guest blogging somewhere ââ¬Ëbigââ¬â¢Ã¢â¬ ¦ 25. Shelley Rodrigo Use others writerââ¬â¢s sentences and paragraphs as models and then emulate the syntactic structure with your own content. Iââ¬â¢ve learned more about grammar and punctuation that way. 26. Sylvia Avoid long sentences. 27. Mike Feeney Learn the difference between me, myself and I. For example: Contact Bob or myself if you have any questions. I hear this very often! 28. Richard Scott When doing a long project, a novel, for instance, shut off your internal editor and just write. Think of your first draft as a complex outline waiting to be expanded upon, and let the words flow. 29. David Careful with unnecessary expressions. ââ¬Å"At this point in timeâ⬠came along during the Nixon congressional hearings. Too bad it didnââ¬â¢t go out with him. What about ââ¬Å"on a daily basis?â⬠30. E. I. Sanchez For large documents, I use Wordââ¬â¢s Speech feature to have the computer read the article back. This allows me to catch errors I have missed especially missing words or words that ââ¬â¢sort of sound the sameââ¬â¢ but are spelled differently (e.g. Front me instead of ââ¬ËFrom meââ¬â¢). 31. Cat Either read the book ââ¬Å"Writing Tools 50 Strategies for Every Writerâ⬠, by Roy Peter Clark, or read the Fifty Writing Tools: Quick List on his blog. Then join a writing group, or hire a writing coach. 32. Suemagoo Write the first draft spontaneously. Switch off your internal editor until it is time to review your first draft. 33. Lydia If youre writing fiction, its a great idea to have a plot. It will coordinate your thoughts and add consistency to the text. 34. Pedro Edit your older articles and pieces. You will notice that great part of it will be crap, and it will allow you to refine your style and avoid mistakes that you used to make. Want to improve your English in five minutes a day? Get a subscription and start receiving our writing tips and exercises daily! Keep learning! Browse the Writing Basics category, check our popular posts, or choose a related post below:Possessive of Proper Names Ending in S"Latter," not "Ladder"Dissatisfied vs. Unsatisfied
Monday, February 17, 2020
Marks and Spencer Financial Statements Coursework
Marks and Spencer Financial Statements - Coursework Example Many of the private companies in the UK use International Financial Reporting Standards (IFRS) conceptual framework for financial reporting (Holland, 1998) and address issues regarding fair value accounting in terms of the 8 phases, out of which four are active: the objective and qualitative characteristics, elements and recognition, measurement, and reporting entity. The conceptual framework itself provides guidance on which measurement bases to be used or outcomes to be obtained through the measurements decisions. The measurements of the different segments are aimed at fair value to meet the qualitative characteristics of the framework. As IFRS includes fair value measurements required by the United Kingdom Generally Accepted Accounting Principles (UK GAAP) (Ballard, 2010, p 704), it is obvious that the different identifiable components (Connolly, 2012) like pension and other associated costs and liabilities of M&S are according to fair value measurement that meets the qualitative characteristics of the framework (Ballard, 2010, p 703). Also, according to the International Accounting Standards Board (IASB) and the Financial Accounting Standards Board (FASB), the objective of fair value measurement is ââ¬Å"to provide guidance for selecting measurement bases that satisfy the objectives and qualitative characteristics of financial reportingâ⬠(FASB, 2009b, p2; Ballard, 2010, p 703). The qualitative characteristics aid financial reporting in the selection of a measurement method which the company has attained through identifying its employees as their valuable asset and also having certain policies and schemes like the pension scheme and work, health and safety schemes as part of its sustainability drive (M&S How We Do Business, 2011). Further, identifiable segments are reportable or operating segments of the company that have reported revenue of more than 10% of the combined revenue of both internal and external revenue of all other segments. Also, if the profit or loss of a particular segment, like employee remuneration or employee benefits, including pension benefits, is greater than profit or loss of all operating segments combined or the assets are worth more than 10% of all combined operating segment assets, then the segment is said to be reportable (Connolly, 2012, p2). This means that M&S has the option of not including the employee remuneration benefits or pension liabilities and costs in the financial report. Measurement is defined in the IASBââ¬â¢s framework as ââ¬Å"the process of determining the monetary amounts at which the elements of financial statements are to be recognized and carried in the balance sheet and income statementâ⬠(IASB, 2001, p 99; Ballard, 2010, p 706). M&S has recognized the measurements relating to employee remuneration, costs, and benefits in its financial statement in 2011 (Appendix 1 & 2). Many of the financial reporting institutes have recognized that there exists ambiguity in standard s attained in financial reporting. There are limitations in financial reporting and public information disclosure as it is common practice with companies to practice mandatory and voluntary public and private disclosure of information that is targeted at specific stakeholders of the business (Holland, 1998).
Monday, February 3, 2020
Evolving Slavery Assignment Example | Topics and Well Written Essays - 1000 words
Evolving Slavery - Assignment Example This is a worldwide event which has existed for hundreds of centuries up to the present. During the ancient times, slavery was brought about by social backgrounds. Slavery was the term used during the ancient period until a certain period when human trafficking was used instead.1 In some cultures, slavery is a hereditary event. Children born to parents who are slaves will automatically be slaves. Thus, their future children will also have the same fate. Slavery has been noted to have occurred at all the ancient civilizations in 4 continents namely Africa, Asia, Europe and pre-Columbian America.2 There is that perception that once a slave, forever that person will remain a slave as well as his or her descendants. It has also been said in an ancient Sumerian code, The Code of Hammurabi, that when a non-slave helps a slave to get out of slavery, the non-slave will be put to death.3 In modern days, as previously stated, slavery is being pertained too, one example of which is human traffi cking.4 With the tradition of slavery being carried through in more recent decades, even if a person is still a child, that person is not free from slavery. However, this is not the only way by which a child becomes a victim of human trafficking. Besides the cultural and traditional factors, poverty is the main reason why there are increasing numbers of children being victimized by human traffickers or syndicates. Cultural influence is one of the factors in human trafficking. Another factor is age. Children can be abducted easier than adults as they are smaller and do not have the physical capabilities and intellectual maturity to fight back against abductors. These factors go together as culture teaches children to obey adults and not fight back. So this also makes abduction easier. Kidnapped children are then enslaved either to do hard works, seek alms on streets or worst be abused and killed.5 In human trafficking, when the victim is said to be a child, it means that it is below the age of 18. The phrase itself evolved from the term slavery by the middle of 20th century. Human trafficking was defined as ââ¬Å"the recruitment, transportation, transfer, harbouring or recipient of persons, by means of the threat or use of force or other forms of coercion, of abduction, of fraud, of deception, of the abuse of power or of a position of vulnerability or of the giving or receiving of payments or benefits to achieve the consent of a person having control over another person, for the purpose of exploitation.â⬠6 On the other hand, slavery has been defined as ââ¬Å"a relationship in which one person is controlled by another through violence, the threat of violence, or psychological coercion, has lost free will and free movement, is exploited economically, and is paid nothing beyond subsistence.â⬠7 It is not just the term which has evolved through the ages. The definition itself has evolved. Bottom line is that whether it is slavery or human trafficking, it is a clear violation of human rights to live freely and improve a personââ¬â¢s way of living. In ancient times, slavery was inherited. There is no way out of slavery, no chance to improve the way of living as if slaves are not human. In recent decades, human trafficking is forcing a person to become a slave. One thing that is common between slavery then and human trafficking now is that both
Saturday, January 25, 2020
White Spot Syndrome Virus (WSSV) Experiment
White Spot Syndrome Virus (WSSV) Experiment Abstract White spot syndrome virus (WSSV) continues to cause huge economic losses in the aquaculture farms due to rapid spread and broad host range. In this study, we synthesized a novel synthetic compound 3-(1-chloropiperidin-4-yl)-6-fluoro benzisoxazole 2 and were screened for antiviral activity against WSSV using fresh water crabs Paratelphusa hydrodomous (Herbst). In vivo bio-assay was carried out to determine the antiviral activity. Reverse transcriptase polymerase chain reaction (RT-PCR) and Histopathology were used for the analysis of bio-assay. Overall result shows that the novel compound has strong antiviral potency against WSSV. Keywords: Paratelphusa hydrodomous, White spot syndrome virus, Synthetic compound, RT-PCR, Histopathology. White spot syndrome virus (WSSV) is a highly pathogenic whispovirus belongs to the family Nimaviridae responsible for causing white spot disease, leads to 100 % mortality within 3-10 days of infection in farmed shrimp (Sudheer et al., 2012). Several antiviral and immunostimulatory compounds are identified from terrestrial plants as well as from the marine origin were tested against WSSV. For example; Sulfated galactans isolated from red seaweed (Gracilaria fisheri) exhibited immunostimulant and resistance against WSSV in Penaeus monodon (Wongprasert et al., 2014), Aqueous extract of Cynodon dactylon showed strong antiviral activity against WSSV in marine shrimp (Balasubramanian et al., 2007). Synthetic compounds like Piperidines and Benzisoxazoles are important group of heterocyclic compounds in the field of medicinal chemistry. These compounds have significant biological and pharmacological properties like anti-inflammatory, antifungal, antimicrobial, and anticancer activities (Gaba et al., 2014; Ramalingan et al., 2004) (Ramalingan, 2004 #21;Gaba, 2014 #22). Many fluorinated, benzisoxazole derivatives are currently used in the treatment of diseases (Prasad et al., 2009). In such a way, there is an immediate need for non-toxic drug to treat WSSV disease. Thus the present study was carried out to determine the antiviral activity and protective effect of a novel synthesized compound 3-(1-chloropiperidin-4-yl)-6-fluorobenzisoxazole 2 (Fig. 1) against WSSV infection in fresh water rice-field crab P. hydrodomous, it was highly susceptible to WSSV (Sahul Hameed et al., 2001). A synthesis of novel 3-(1-chloropiperidin-4-yl)-6-fluorobenzisoxazole was carried out under mild reaction conditions using 1.2 equivalents of calcium hypochlorite. Interestingly, the developed method does not involve any additives like acids or bases and provides 96 % of isolated yields at room temperature. This novel molecule, 3-(1-chloropiperidin-4-yl)-6-fluorobenzisoxazole 2 was stable at ambient conditions and stereo chemistry was established the single crystal XRD technique. The materials were purchased from Sigma-Aldrich, Merck and were used without any additional purification. All reactions were monitored by thin layer chromatography (TLC). Melting points were recorded on an Elchem digital melting point apparatus in open capillaries and are uncorrected. The 1H NMR was measured on a Bruker Avance-400 MHz instrument at room temperature. The 1H NMR was measured for ~0.03 M solutions in CDCl3 using TMS as internal reference. The accuracy of the 1H shifts is considered to be 0.02 p pm. The coupling constants J are in Hertz. Mass spectra were obtained using ESI mass spectrometry. 6-fluoro-3-(piperidin-4-yl) benzisoxazole 1 (5 g, 22.7 mmol) was taken into the round bottom flask dissolved in 50 mL of acetonitrile. To this calcium hypochlorite (3.9 g, 27.3mmol) was slowly added over ten to twenty minutes. Reaction was monitored by TLC. After the reaction completion, reaction mass was filtered and salts was washed with acetonitrile. Solvent was dried under reduced pressure. Crude solid was purified by column chromatography to give 3-(1-chloropiperidin-4-yl)-6-fluorobenzisoxazole 2 in 96 % (5.54g, 21.8mmol) as pale yellow color solid. The structure of the N-chloro benzisoxazole was conà ¯Ã ¬Ã rmed from their spectral data from NMR, ES Mass and single crystal XRD. Mp: 81-83oC; 1H NMR (CDCl3, 400 MHz) d (ppm): 7.71-7.08 (m, 3H), 3.65 (d, 2H), 3.22 (t, 3H), 2.36-2.15(m, 4H); 13C NMR (CDCl3, 100 MHz) d (ppm): 165.3, 163.9, 162.8, 159.9, 122.6, 122.2, 122.1, 116.9, 112.4, 97.6, 97.4, 32.9; MS (ESI) m/z Calcd: 254.1, found: 253 (M-1); Single crystal crystal data 3-(1-chloropiperidin-4-yl)-6-fluoro benzisoxazole 2 Mol. Formula:C12H12ClFN2O; CCDC reference number is 878706; Intensity data were collected on an APEX CCD diffract meter equipped with Moââ¬âKa (l = 0.7107 Aà °) radiation; Cell length a =5.8979(4); Cell length b=10.4965(7); Cell length c=19.1492(12); Cell Angle à ±=90.0; Cell Angle à ²=91.783; Cell Angle à ³=90.0; Cell Volume=1184.90(11); The crystallographic data for N-chloro benzisoxazole have been deposited with the Cambridge Crystallographic Data Centre. Copies of this information may be obtained free of charge from the Director, CCDC, 12 Union Road, Cambridge, CB2 1EZ, UK [Fax: 44(1223)336033, or http:// www.ccdc.cam.ac.uk]. The crabs P. hydrodomous (20-25 g body weight) were collected from the rice field located at kalavai, Vellore, India. Crabs were transported to the laboratory. A previous method was followed for maintaining the crabs and preparation of WSSV inoculum (Nambi et al., 2012). For in vivo determination of antiviral activity, the healthy crabs were divided into three groups contains 3 crabs per group and each trial was conducted in triplicates. Crabs in the group I were injected with 100 à µl of a mixture of viral suspension and NTE buffer which served as positive control. In Group II crabs were injected with NTE buffer alone served as negative control. In Group III crabs were injected with viral suspension, novel compound and NTE buffer served as treated. The viral suspensions for all groups were incubated at room temperature for 3 h. Later it was injected into respective experimental groups intramuscularly. The experimental animals were examined twice per day for gross signs of disease, and the number of deaths was recorded until end of the experiment. Animals in the treated and negative control group were survived without any mortality and sign of WSSV infection until end of the experiment. Whereas the positive control group reached 100 % mortality at 7th day of post injection with gross signs including reduced feed consumption, less active in slow in movement. The observation of this bio-assay was plotted in a cumulative mortality graph (Fig. 2). Hemolymph from all the 3 groups was collected for hematological analysis (Total hemocyte count and clotting time). In positive control, there were significant reduction in total hemocyte counts as well as the hemolmph was failed to clot. No significant hematological changes were observed in between the negative and treated groups. For RT-PCR analysis; Gill, head-soft tissue, heart and muscle tissue were excised from each crabs of the experimental group and pooled together for extraction of total RNA using Trizol (Invitrogen, USA) according to the manufacturers instructions. cDNA was synthesized from 1.0à ¼g of the total RNA using a One-step Reverse Transcriptase (Invitrogen, USA) as per the kit instructions and used as template for gene expression analysis of WSSV specific primer VP28. à ²-actin served as an internal control for RNA quality and amplification efficiency. The sequences of primers used in this present study were given in Table 1. The cycling conditions are initial denaturation at 95à °C for 5 min followed by 35 cycles of denaturation at 95à °C for 30 sec, annealing at 50à °C for 30 sec and extension at 72à °C for 30 sec with a final extension at 72à °C for 10 min. The amplified PCR products were electrophoresed in 1.0 % agarose gel stained with ethidium bromide and visualized by ultraviole t transilluminator. There were no bands was found for negative control and all the tissue cDNA templates from the treated crabs, a band came at 615 bp for positive control (Fig. 3A). Bands came well for the same templates subjected to à ²-actin PCR (Fig. 3B). For histological analysis, a small portion of gill and head-soft tissue was taken from all the three experimental groups and was fixed in Davidsonââ¬â¢s fixative for subsequent histological preparations (Bell and Lightner, 1988) with haematoxylin and eosin according to the standard protocol. The stained sections of gills and head-soft tissue from the control crabs show no histopathological changes (Fig. 4A 4B). Whereas in the positive control cells having hypertrophied nuclei with intranuclear inclusions typical for WSSV infection (Fig. 4C 4D). No significant changes were observed in treated group (Fig. 4E 4F) indicates no WSSV infection. In conclusion, a novel compound 3-(1-chloropiperidin-4-yl)-6-fluorobenzisoxazole 2 derivatives showed strong antiviral activity against WSSV in fresh water crabs P. hydrodomous. This works may help to design a novel non-toxic drug to treat WSSV infection. Acknowledgements References Balasubramanian, G., Sarathi, M., Kumar, S.R., Hameed, A., 2007. Screening the antiviral activity of Indian medicinal plants against white spot syndrome virus in shrimp. Aquaculture 263, 15-19. Bell, T.A., Lightner, D.V., 1988. A handbook of normal penaeid shrimp histology. World aquaculture society, Baton Rouge, LA. Gaba, M., Singh, S., Mohan, C., 2014. Benzimidazole: An emerging scaffold for analgesic and anti-inflammatory agents. Eur. J. Med. Chem. 76, 494-505. Nambi, K.N., Majeed, S.A., Raj, N.S., Taju, G., Madan, N., Vimal, S., Hameed, A.S., 2012. In vitro white spot syndrome virus (WSSV) replication in explants of the heart of freshwater crab, Paratelphusa hydrodomous. J. Virol. Methods 183, 186-195. Natividad, K.D.T., Nomura, N., Matsumura, M., 2008. Detection of White spot syndrome virus DNA in pond soil using a 2-step nested PCR. J. Virol. Methods 149, 28-34. Prasad, S.B., Vinaya, K., Kumar, C.A., Swarup, S., Rangappa, K., 2009. Synthesis of novel 6-fluoro-3-(4-piperidinyl)-1, 2-benzisoxazole derivatives as antiproliferative agents: A structureââ¬âactivity relationship study. Invest. New Drugs 27, 534-542. Ramalingan, C., Balasubramanian, S., Kabilan, S., Vasudevan, M., 2004. Synthesis and study of antibacterial and antifungal activities of novel 1-[2-(benzoxazol-2-yl) ethoxy]-2, 6-diarylpiperidin-4-ones. Eur. J. Med. chem. 39, 527-533. Sahul Hameed, A., Yoganandhan, K., Sathish, S., Rasheed, M., Murugan, V., Jayaraman, K., 2001. White spot syndrome virus (WSSV) in two species of freshwater crabs (Paratelphusa hydrodomous and P. pulvinata). Aquaculture 201, 179-186. Sudheer, N., Philip, R., Singh, I.B., 2012. Antiââ¬âwhite spot syndrome virus activity of Ceriops tagal aqueous extract in giant tiger shrimp Penaeus monodon. Arch Virol. 157, 1665-1675. Wongprasert, K., Rudtanatip, T., Praiboon, J., 2014. Immunostimulatory activity of sulfated galactans isolated from the red seaweed Gracilaria fisheri and development of resistance against white spot syndrome virus (WSSV) in shrimp. Fish Shellfish immunol. 36, 52-60. Table 1 Primers used for the RT-PCR Primer name Sequence (5ââ¬â¢- 3ââ¬â¢) Annealing temperature Product size VP28-F ATG GAT CTT TCT TTC AC VP28-R TTA CTC GGT CTC AGT GC 50à ¯Ã¢â¬Å¡Ã °C 615 bp à ²-actin-F à ²-actin-R GTG CCC ATC TAC GAG GGA TA GTG TTG GCG TAC AGG TCC TT 55à ¯Ã¢â¬Å¡Ã °C 404 bp Fig. 1. Single crystal ORTEP diagram of the 3-(1-chloropiperidin-4-yl)-6-fluoro benzisoxazole 2 Fig. 2. Cumulative mortality graph for the experimental groups. Fig. 3. (A) RT-PCR of WSSV envelope protein VP28 in different organs of treated group. Lane 1, 100 bp DNA marker; 2, WSSV positive control; 3, negative control; 4, gill; 5, head-soft tissue; 6, heart; 7, muscle tissue. (B) RT-PCR results of the same samples for à ²-actin gene. Fig. 4. Photomicrographs of tissue from crabs of experimental groups: 4A gill and 4B head-soft tissue of negative control showing normal cells (Arrow); 4C gill and 4D head-soft tissue of positive control showing hypertrophied nuclei with intranuclear inclusions (Arrow); 4E gill and 4F head-soft tissue of treated group showing uninfected (Arrow). Original magnification: 1000 X.
Friday, January 17, 2020
Judging on Apperance
Justin Tipton What is the first thing we do when meeting a first. We judge! Judging someone based on their appearance is an inherent human instinct. Many times when people judge a person, people end up making wrong decisions. We create mental images in our minds that categories people based upon their appearance. When you see a man in a suit, tie, and a briefcase you figure is a lawyer, professor, or a businessman. In reality the guy that you see could have been out for a special event and could be no more than a car mechanic. We base our opinions on perceptions on people based upon what we can biologically see with our eyes.In her article how the eye works Ker Than explain how we use our eyes to visually see things. ââ¬Å"Vision begin when lights rays are reflected off an object and enter the eyes through the cornea, The transparent outer covering of the eyes. The cornea refracts the rays that pass through a round hole, called the pupil. The Irish is the colored portion of the eye that surrounds the pupil, and then become larger or smaller to regulate the amount of light passing through. The rays then travel through the lenses to the retina at the back of the eye. The retina conducts light into electrical impulses.The optic nerve sends impulses to the brain where a visual image or perception is produced. â⬠We use our eye to visually see people, and to make an array of judgment about that person based on what we see. While itââ¬â¢s true we can come times tell certain thing about people by looking at their external appearance, it is not the best of measurements. I believe people should not judge people by their external appearance, and should at least speak with a person before making a judgment. You canââ¬â¢t judge a person character base on appearance and that why many of us make unfair and bias assumption of others.People sometimes change their appearance to fit different situations. According to a Washington post article, Joshua bell one of the world greatest violinists set out to do an experiment to test peopleââ¬â¢s perception on appearance. By most, he was nondescript: a youngish white man in jeans, a long sleeved t-shirt and Washington National baseball cap. He positioned himself against a wall beside a trash basket, from a case; he moved his violin and began to play. It was a Friday and the middle of morning rush hour. The violinist played for nearly an hour, and performed six classical pieces.Nearly 1,100 people passed by, many of them on their way to work. No one knew it, but the fiddler standing against a bare wall outside was one of the finest classical musicians in the world, playing some of the most elegant music ever written on one of the most valuable violins ever made. In the hour that Joshua Bell played, seven people stooped what they were doing to hang around and take in the performance, at least for minute. Twenty-seven gave money, for a total of thirty-two dollars and seventeen cents. That means over 1 ,070 people passed on by, with few even turning to look.Three days before conducting this experiment on a street corner in Washington, Joshua bell filled the house at Bostonââ¬â¢s stately symphony Hall, where the average seat went for one-hundred dollars. the appearance of a guy in plain clothes playing a violin at the street corner, made many people ignore his sound before ever even taking the time to listen. Many times people judge what they see, and ignore the beauty that right in front of their face. To begin with, someone external appearance is not mirror of oneââ¬â¢s inner self. I can relate to from examples in my own life.Being a young African-American male, growing up near Memphis one of Americas most dangerous cities. I came from a poorer family, I been working full time since attending college. In the day time I was a college student, but at work I was a normal sweaty and dirty factory worker. When people saw me at work, they assumed I was uneducated and not very int elligent. In reality, I was a good academic student, has a good reputation among my fellow students and professors. My co-workers couldnââ¬â¢t see the college side of me, and many of my fellow students couldnââ¬â¢t see working in a factory which was known for harsh working conditions.Since leaving the factory, I know have a security job just outside of downtown Nashville. TRICOR is the name of the corporation is profits in the million, by utilizing inmates for production and paying their cheap labor wages. This cooperation is ran by your typical CEO, a short old white man, that dresses in suits and ties and owns many expenses vehicles. For many months while working there, he passed by me every day and never once he even acknowledging my presence. One day I was reading a book about written from a famous entrepreneur, and the boom caught the eye of the CEO.He walked up to me and asked why I reading a book like that, I told I was a college student that was a business major, and t hat it has beneficial information in this nook, and that I read many book from this author. He then brought to my attention that he has read the same book and he too was a fan of the author books. After conversation for a few minutes he then brought to my attention that he too grew up from a poor family, and that he also work at a factory and once at a security job while too attending college. I am a business management major and he too has a degree in business management .Ever since that day of our conversation he speak to me daily, and we hold brief but interesting conversation often. Prior to that conversation we had never spoken to another, once we looked past the external appearance, we realized we two men who shared many things in common. You may not realize it, but perception based on appearance happen all the time. Studies show that fifty-five percent of opinion of a person is based upon physical appearance, and that it takes around fifteen second for a person to realize if he or she like your or not, or want to do business with you.Do honestly we feel like we get accurate of someone inner self with over half of what we perceive come from appearance, and while only judging them for one-fourth of a minute? Many people believe that judging people on the appearance is beneficial and effective. Mangers often use appearance as a asset while evaluating a candidate for a job. You could have two good candidates with impressive resume, but choosing a candidate based on race, or which dressed too me is ineffective. In reality just because one candidate is dressed better, doesnââ¬â¢t make him any smarter or better qualified than the other.When it comes to judging a lover or friend, external appearance shouldnââ¬â¢t be a factor on your judgment about that person. Much time we see that a man or women date someone because she is beautiful and he is handsome. In high school and college you see on many occasions when someone hangs around a certain person because it will increase his/her popularity. In a poem I once read it state ââ¬Å"we see best when we look for the lessâ⬠. In just a few words, that poem speaks a lot, we see the best when look past the artificial obvious, and see the beauty that deep within a person.Beauty and the Beast just a fairy tale and a kidââ¬â¢s story but moral of this tale has such a deeper meaning. The story about a beautiful women falling in love with an ugly beast, passed artificial obvious and see the beauty that lied deep within him. Making a judgment on some based only external appearance is an unfair and accurate judgment of someone. It is not accurate to judge someone based on we biologically see wit our eyes. A appearance can adjust and change their appearance to fit many different situation where there true selves do not show, but a person cannot change his/her character.For these reason, it is important to get to know someone before making a judgment. ââ¬Å"The best and most beautiful things in the world cannot be seen or touchedâ⬠. ââ¬Å"But must be felt by the heartâ⬠. The best and most beautiful things in people cannot be seen by the eyes. Judging someone on external appearance is an inherent and instinct that happen almost instantaneously. We as people have the ability to control our judgment, we have the ability to use our minds to see more within people than what we see with our eyes.Stero-typing is something we do without even noticing, but placing people into different categories based on external appearance limits our ability to meet new people. Sometimes the people that we expect knot to have anything in common with, could be a totally false assumption. If we allow ourselves to judge and meet people of different culture, many would be surprised to know they share many things in common with people they would have never suspected. As long as people allow ourselves to be blinded by external appearance then we will fully see the inner beauty within so m any people.
Thursday, January 9, 2020
Needle Cast Tree Disease Prevention and Control
Needle cast is a broad group of fungal diseases that cause conifers to shed needles. The symptoms of needle cast first appear on needles as light green to yellow spots, which eventually turn red or brown. Growth of the fungal pathogen from the spots on the needle will cause the death of the entire needle. This shedding of needles can be more serious to conifers than losing leaves is to deciduous hardwoods. There are over 40 kinds of needle casts in North America. Recognition Infected needles usually turn red to brown from their tips beginning in winter or early spring. By mid to late spring the death of infected needles is well advanced giving diseased trees a red to brown fire-scorched appearance. Tiny black fruiting bodies (spore producing structures) form on the surface of the needles before or after the infected needles are shed. Prevention Avoid planting trees on sites not suited for a particular species. Needle cast seems to thrive when conifers are under stressful conditions including drought. Young seedlings and saplings are susceptible, as well as pure and crowded stands. Keeping your tree healthy can minimize the harmful effects of this disease.Ã Control Control is unnecessary in most non-commercial situations. However, Christmas tree growers must take some action against the disease. If control is desired for cosmetic reasons, protection of newly emerging needles through June with regular applications of an appropriate fungicide may be helpful.
Wednesday, January 1, 2020
Pediatric Cancer Is A Scary Concept - 1874 Words
Pediatric Cancer is a scary concept. We think as a society that children are innocent and should not have to go through such a deadly and scary adult disease. Unfortunately that is not true. Cancer has no limits or prejudices on what the age, sex, or race of its victim will be. With this paper I want to discuss multiple topics about pediatric cancer. First the statistics, then the treatment options, finally the treatment/prognosis for the family as a whole and not just the patient. There are different treatments that should occur when dealing with the family members of a patient that has cancer. A social worker that works in pediatric oncology truly wears many hats. Statistics In 2014 it was estimated that approximately 16000 children were diagnosed with cancer and approximately 2000 will die from the disease.(nih) However in 2010 it was estimated that there are almost 400,000 survivors that were diagnosed before the age of 19. That is amazing news. Almost half a century ago the outlook was not so bright almost 50% of children diagnosed with cancer would die within 5 years now there is an 80% survival rate. There are different rates for different types of cancer. The most common type of pediatric cancer that is diagnosed involves brain tumors and blood cancers. The mortality rates have greatly improved for leukemia going from 10% to 90% survival rates since the early 1970ââ¬â¢s. On the flip side there are still some forms of cancer such as diffuse pontine glioma (brainShow MoreRelatedThe Definition Of Nursing And Share My Thoughts On What Makes The Ideal Nurse Essay1505 Words à |à 7 Pagesmy way of giving back and helping those in my community. I t ruly enjoy working with children and believe I can make a difference in each patientââ¬â¢s life. I ultimately decided to join the profession of nursing after my experience with cancer. Battling cancer was scary, but my nurses and physicians truly fought to keep me alive. During this experience I felt the nurses saw me as a human being and not just ââ¬Å"another patient.â⬠My treatment focused on the mind, body, and spirit aspect and I do believeRead MoreA Little More About Gender And Sex1014 Words à |à 5 PagesHunter-Angel Gregory Mrs. Williams ELA 3/4th Hour 7 April 2017 A LITTLE MORE ABOUT GENDER SEX One thing people have thought until recently is that the concepts of gender and sex are the same thing. This is now something that is changing and proving to be false. That being said, they can be troublesome to learn. Acceptance is another tough thing people now deal with since having these thoughts shared with them. Once upon a time the earth was thought to have beenRead MoreOrganizational Leadership and Interprofessional Team Development10384 Words à |à 42 Pagesit comes to your health, you can feel confident knowing that the new MCHS is your one source for patient and family centered care as the hospital system has developed a full service Integrated delivery system, services offered are listed below: ï⠧ Cancer ï⠧ Cardiology ï⠧ Cauterization Lab ï⠧ Chest Pain Center ï⠧ Cardiac Rehab ï⠧ Electrophysiology ï⠧ Cardiac Surgery ï⠧ Community Outreach ï⠧ A Tu Salud (To Your Health) ï⠧ Permian BasinRead MoreToo Far Ahead of the It6117 Words à |à 25 Pagespoint. Eventually, he always fell back on his clinical experience. ââ¬Å"You can standardize the testing of ball bearings for manufacturing defects,â⬠he said. ââ¬Å"But as far as I know, you canââ¬â¢tââ¬âat least not yetââ¬âstandardize the protocol for treating colon cancer.â⬠As a physician, Max believed that the last word in all matters of patient care should rest with the doctor and the patient. But as a CEO he believed in best practices. So his compromise position was to favor selective (Max called it ââ¬Å"surgicalâ⬠)
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